Description: LDS Sample Buffer, Non-Reducing (4X), 5 ml, contains 40% glycerol, 4% lithium dodecyl sulfate (LDS), 0.8 M triethanolamine-Cl pH 7.6, 4% Ficoll®-400, 0.025% phenol red, 0.025% coomassie G250, 2 mM EDTA disodium. 

Storage: Upon receipt store at room temperature.

Introduction: The LDS Sample Buffer, Non-Reducing (4X) is a convenient sample buffer for use in SDSpolyacrylamide gel electrophoresis (SDS-PAGE). The buffer contains coomassie dye, enabling visualization of the electrophoresis progress by the location of the dye front. The LDS Sample Buffer, Non-Reducing (4X) may be used in denaturing gels and is compatible with coomassie dye and silver staining, and Western blotting procedures.

Procedure:   

Note: For reducing gels, add an appropriate reducing agent to the sample before electrophoresis. 

 1. Mix one volume of LDS Sample Buffer with three volumes of protein sample (e.g., 5 μl sample buffer 15 μl protein sample). Note: Failure to properly dilute the sample buffer may cause a diffuse dye front. 

 2. Heat sample at 100°C for 3-5 minutes. 

 3. Allow sample to cool to room temperature. 

 4. Load sample onto the SDS-polyacrylamide gel and begin electrophoresis. 

 5. Stop electrophoresis when the dye front is near the bottom of the gel.